"This wasn't what I wanted"

You might wonder how you ended up here, which looks very different. The reason, a simple one, is that I have now (largely) retired, and this move is to ensure that my buffer calculator, as a few other bits and pieces, survive as I step away from the University, and from academic life in the large.

The buffer calculator has amassed about one and a quarter million hits, and seems to be valuable to the community, so I will keep it going, and hopefully, pass the code to another to continue to maintain and update as time goes on. Please make a note of the new link, phbuffers.org from now on, and change any redirects to links you might have used.

Thanks!

Rob


p.s. Could you take a moment to read the entreatment on the buffers page?




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A million litres!
At about 8pm on January 27th, 2020, my on-line buffer calculator broached the one million hits mark.

A million litres of buffer is the same volume as in the AquaDom in Berlin.
This is my personal website.

In 2020 I formally retired from the University of Liverpool (although I maintain research activity until mid 2022).

With the move away from the University, there were some things that would have been lost. First, the group website is being transitioned to a more corporate site, and I wanted to retain some of the features of the old site.

Secondly, the group site hosted my calculator page for the preparation of thermodynamically correct buffers for pH control. This continues to be very popular. It is now hosted here.

Recent publications


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ALACATs
QconCATs have amassed well over 1,200 citations, and are widely known and understood (so much so, that the original papers are no longer cited!). But, they are not that easy to deploy. We have made some changes, including cell-free biosynthesis and now, a synthetic biology approach that allows us to assemble a QconCAT to order, from a library of quantotypic peptide-encoding oligoonucleotides. It is now possible to build a permanent library of olives that can be assembled in an 'a la carte' assembly, to make the desired QconCAT, or any length. The ALACAT approach also makes it easy to replace poorly-performing peptides. [Paper here]
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Catnip!
No, not yet another dodgy titled paper with an acronym of QconCATnip! This is a collaboration with colleagues in Iwate University, showing that cats anoint themselves with catnip to repel mosquitoes. [here]





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Gels and MS still have their place. A new paper with our colleague and friend Nobuaki Takemori, showing just how much information can be recovered by MS of gel-recovered proteins. [here]
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Sperm competition
Two new papers on our work with MBE - one on seminal vesicle fluid plasticity and the other using stable isotopes to track relative ejaculate investment in voles.



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QconCAT on YouTube
View a short YouTube video that explains how to use QconCATs for isoform quantification [
Video]

PLus, if you want to know what it is like working in CPR, see [
this interview with Rosie].
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Web resources for proteomics
Pastel Biosciences have compiled an impressive set of web resources for proteomics. [HERE]

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Posters of our work
CPR always prepared a full sized poster that covers the work we published: [
2014] [2015] [2016] [2017][2018][2019]. We also publish an annual summary of great events. [EVENTS 2015] [EVENTS 2016] [EVENTS 2017][EVENTS 2018][EVENTS 2019]. The 2020 posters will appear here at the end of the year.

Other news


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CPR Datasets in PRIDE.
We are working hard to support open access and open data initiatives, and deposit raw data in [
PRIDE]. You can find our submitted datasets elsewhere on the ProteomeExchange site [HERE]. If you want to see why making data available is important, look [here] and [here], in that order.
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Annual CPR ‘Year in review” posters
At the end of each year we produce a poster that captures our research outputs for the year, and also collates all of our main events, outreach, STEM activities etc. If you’d like to see these posters, then look for [EVENTS 2018] and [PUBS 2018] (PDF files). You can find our papers here (main menu, Papers) and pick up news information here.