Stable Isotopes

March of the acronyms.. ALACATs (a la carte proteomics)

James Johnson, Victoria M Harman, Catarina Franco, Edward Emmott, Nichola Rockliffe, Yaqi Sun, Lu-Ning Liu, Ayako Takemori, Nobuaki Takemori, Robert J Beynon (2021) Construction of à la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins (2021) BMC Biol 2021 Sep 8;19(1):195. doi: 10.1186/s12915-021-01135-9. [PDF] [PUBMED] [DOI] [Abstract...]

Using stable isotopes to track sperm competition

Paula Stockley, Catarina Franco, Amy J. Claydon, Amanda Davidson, Dean E. Hammond, Philip J. Brownridge, Jane L. Hurst, and Robert J. Beynon (2020) Revealing mechanisms of mating plug function under sexual selection. [PDF] [PUBMED] [DOI] [Abstract...]

A new lipocalin, fully sequenced, from bank voles

Loxley GM, Unsworth J, Turton MJ, Jebb A, Lilley KS, Simpson DM, Rigden DJ, Hurst JL, Beynon RJ. (2017) Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus. [PDF] [PUBMED]

Proteome dynamics in a non-model species - the challenges!

Hammond DE, Claydon AJ, Simpson DM, Edward D, Stockley P, Hurst JL, Beynon RJ. (2016) Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals. [PUBMED] [PDF] [Abstract...]

The biggest absolute quantification study ever! A tale of 100 QconCATs for nearly 2,000 proteins

Lawless C, Holman SW, Brownridge P, Lanthaler K, Harman VM, Watkins R, Hammond DE, Miller RL, Sims PF, Grant CM, Eyers CE, Beynon RJ, Hubbard SJ. (2016) Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. [Abstract...]

Designing QconCATs for a mammalian pathway

Simpson, D.M. & Beynon, R.J. (2012) QconCATs: design and expression of concatenated protein standards for multiplexed protein quantification. [PUBMED][PDF] [Abstract...]

Whole animal protein turnover studies

Claydon, A.J., Thom, M.D., Hurst, J.L. & Beynon, R.J. (2011) Protein turnover: measurement of proteome dynamics by whole animal metabolic labelling with stable isotope labelled amino acids. Proteomics 12:1194-1206 [PUBMED][PDF] [Abstract...]

Positional proteomics and MetAP-2 substrates

Thomas B. Sundberg, Nicole Darricarrere, Pasquale Cirone, Xia Li, Lucy McDonald, Xue Mei, Christopher J. Westlake, Diane C. Slusarski, Robert J. Beynon, and Craig M. Crews (2011) Disruption of Wnt Planar Cell Polarity Signaling by Aberrant Accumulation of the MetAP-2 Substrate Rab37. [PUBMED][PDF] [Abstract...]

Enzyme turnover and metabolic regulation

Arden, C, Petrie, J.L., Tudhope, S.J., Al-Oanzi, Z., Claydon, A.J., Beynon, R.J., Towle, H.C. & Agius, L. (2011)Elevated glucose represses liver glucokinase and induces its regulatory protein to safeguard hepatic phosphate homeostasis [PUBMED][PDF] [Abstract...]

FTICR based quantification

Johnson, H., Wong, S C C, D M Simpson, R J Beynon, and S J Gaskell (2008) . Protein quantification by selective isolation and fragmentation of isotopic pairs using FT-ICR MS. J Am Soc Mass Spectrom 19, 973-977 [PUBMED][PDF] [Abstract...]

Proteomics of HGF/EGF signalling

Hammond DE, Hyde R, Kratchmarova I, Beynon RJ, Blagoev B, Clague MJ. (2010) J Proteome Res. 9(5):2734-2742. Quantitative analysis of HGF and EGF-dependent phosphotyrosine signaling networks.[PUBMED][PDF] [Abstract...]